Postbiotic oral lozenges: a four-week human study

Seventy-five healthy adults took postbiotic, heat-killed probiotic or placebo lozenges. The two active preparations differed and must be reported separately.

Human lozenge study · 2022 · Aging-US

What was tested?

Relationship to the ingredient
Research on the PE0301 source-strain formulation
Study design
Randomized Controlled Trial
Participants or model
75 adults
Duration
4 weeks

Reported findings

  • Laboratory assay:95.32~100% inhibition of P. gingivalis and 87.88% inhibition of F. nucleatum in in-vitro testing. These are laboratory results, not measurements taken in participants.
  • Human study:At week 4, salivary Lactobacillus reached 227% of baseline in the postbiotic lozenge group.
  • Human study:At week 4, salivary IgA reached 168% of baseline in the postbiotic lozenge group.
  • Human study:Participants in both active groups reported fewer oral symptoms such as mouth sores, and some reported changes in constipation and reflux. These are subjective questionnaire responses.
  • Human study:NGS confirmed L. salivarius enrichment after 4 weeks (0.03% with the postbiotic lozenge; 0.06% with the heat-killed lozenge), both significantly higher than placebo.

What the findings do not establish

The trial ran for four weeks in healthy adults and measured microbial and immune markers. It does not establish treatment of dental or gastric disease. Results apply to the tested lozenge preparations at the doses used, and the two active preparations must be reported separately.

Extended study summary

Study summary: This four-week randomised study enrolled 75 healthy adults, assigned to three groups of 25: postbiotic lozenges derived from Lactobacillus salivarius subsp. salicinius AP-32, L. paracasei ET-66 and L. plantarum LPL28; heat-killed probiotic lozenges of AP-32 and ET-66; or placebo. Researchers measured oral bacteria and salivary immune markers, and collected questionnaires on oral and intestinal symptoms. Both active groups showed increases in salivary immunoglobulin A and in selected beneficial oral bacteria compared with placebo. Antimicrobial activity against oral pathogens was measured in separate laboratory assays, not in the participants themselves. The two active preparations differ in composition and should not be treated as interchangeable.

Original publication

Impact of the food grade heat-killed probiotic and postbiotic oral lozenges in oral hygiene

Lin, C. W., Chen, Y. T., Ho, H. H., Kuo, Y. W., Lin, W. Y., Chen, J. F., Lin, J. H., Liu, C. R., Lin, C. H., Yeh, Y. T., Chen, C. W., Huang, Y. F., Hsu, C. H., Hsieh, P. S., Yang, S. F.

Aging-US, 2022

Read the original paper: 10.18632/aging.203923

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